Page 23 - HKSEMR2020 Programme book

Poster Presentation (Basic Science) Abstracts

µM) on PDIA1 expression. The Jeg-3 spheroid attachment rate is Conclusion:
significantly higher in receptive (Ishikawa & RL95-2) than non- Our findings suggest that estrogen, progesterone and their
receptive (AN3CA & HEC1-B) cells. Inhibition of PDIA1 by bacitracin receptors modulate PDIA1 expression on endometrial epithelial
(1mM) or knockdown of PDIA1 by siRNA increased Jeg-3 spheroid cells and endometrial receptivity. The increased attachment was
attachment on non-receptive AN3CA cells, whereas forced exerted partly through up-regulation of adhesion molecule integrin
expression of PDIA1 reduced the attachment in receptive Ishikawa β3 and a reduced microenvironment for embryo attachment.
cells. Furthermore, forced expression of PDIA1 in Ishikawa cells Screening for membrane PDIA1 expression before embryo transfer
significantly down-regulated expression of cell adhesion molecule in infertile women may open a new approach to enhance embryo
integrin β3, but not integrin αv and E cadherin. Interestingly, implantation in clinical setting.
disulphide reducing TCEP (1 mM) treatment increased spheroid
attachment in AN3CA, but not in Ishikawa cells. Conversely, [The project is partly funded by HMRF grant 15162211 to KFL]
oxidizing reagent diamide (0.1 mM) treatment reduced Jeg-3
spheroid attachment in Ishikawa, but not in AN3CA cells.

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